Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Adenosine triphosphate | 5 mM, 1.55fold activation | Methylomonas methanica | |
glyceraldehyde-3-phosphate | slight activation | Methylomonas methanica | |
phosphoenolpyruvate | 5 mM, 1.11fold activation | Methylomonas methanica | |
ribose-5-phosphate | 5 mM, 1.18fold activation | Methylomonas methanica |
Cloned (Comment) | Organism |
---|---|
heterologous expression in Esherichia coli | Methylomonas methanica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | 0.5 M, 7% loss of activity | Methylomonas methanica | |
AMP | 0.5 M, 16% loss of activity | Methylomonas methanica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
(phosphate)45 | 28°C, pH 8.5 | Methylomonas methanica | |
0.45 | - |
D-glucose | 28°C, pH 8.5 | Methylomonas methanica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
6000 | - |
gel filtration, dimerization in presence of polyP | Methylomonas methanica |
30000 | - |
SDS-PAGE | Methylomonas methanica |
30000 | - |
gel filtration in absence of polyP | Methylomonas methanica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(phosphate)n + D-glucose | Methylomonas methanica | - |
(phosphate)n-1 + D-glucose 6-phosphate | - |
? | |
(phosphate)n + D-glucose | Methylomonas methanica 12 | - |
(phosphate)n-1 + D-glucose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylomonas methanica | A0A3G4RHV5 | - |
- |
Methylomonas methanica 12 | A0A3G4RHV5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methylomonas methanica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(phosphate)45 + D-glucose | - |
Methylomonas methanica | (phosphate)44 + D-glucose 6-phosphate | - |
? | |
(phosphate)45 + D-glucose | - |
Methylomonas methanica 12 | (phosphate)44 + D-glucose 6-phosphate | - |
? | |
(phosphate)n + D-glucose | - |
Methylomonas methanica | (phosphate)n-1 + D-glucose 6-phosphate | - |
? | |
(phosphate)n + D-glucose | - |
Methylomonas methanica 12 | (phosphate)n-1 + D-glucose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE, monomer oder dimer, dimerization in in presence of polyP | Methylomonas methanica |
Synonyms | Comment | Organism |
---|---|---|
PolyP-dependent glucokinase | - |
Methylomonas methanica |
polyP-Glk | - |
Methylomonas methanica |
polyphosphate-dependent glucokinase | - |
Methylomonas methanica |
PPGK | - |
Methylomonas methanica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Methylomonas methanica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
2 h, 30% loss of activity | Methylomonas methanica |
35 | - |
2 h, 64% loss of activity | Methylomonas methanica |
40 | - |
2 h, 95% loss of activity | Methylomonas methanica |
55 | - |
30 min, complete inactivation | Methylomonas methanica |
60 | 65 | 5 min, complete inactivation | Methylomonas methanica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0066 | - |
D-glucose | 28°C, pH 8.5 | Methylomonas methanica | |
0.0068 | - |
(phosphate)45 | 28°C, pH 8.5 | Methylomonas methanica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Methylomonas methanica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10.5 | active at a pH range of 5.5-10.5 | Methylomonas methanica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0145 | - |
D-glucose | 28°C, pH 8.5 | Methylomonas methanica | |
0.33 | - |
(phosphate)45 | 28°C, pH 8.5 | Methylomonas methanica |